Solution I Potassium Ferrocyanide, 4%
Solution II Hydrochloric Acid, 4%
Solution III Phenolated Basic Fuchsin

CONTENTS: Potassium Ferrocyanide, HCl, Safranin O Potassium Ferrocyanide, HCl, Basic Fuchsin, Phenol, Ethanol

These solutions are made from certified dyes (when applicable).

FOR IN VITRO DIAGNOSTIC USE ONLY.

RECOMMENDED PROCEDURE: Working Solution: Just prior to use, mix equal parts of Solution I with Solution II. Fix slide in formalin vapor by placing in a closed staining jar containing a sponge or a piece of filter paper slightly moistened with formalin for 2 - 3 minutes. Slide is placed in the working solution that has been heated to 56oC or 132oF. Slide remains in working solution for 30 minutes and then removed and rinsed in tap water. Slide is then counterstained in Solution III - Basic Fuchsin solution for 5 minutes. Rinse slide in tap water, then ethanol and then again in water. Allow to air dry.

NOTE: The use of chemically clean glassware and plastic-tipped or paraffin-coated forceps is suggested.

RESULTS: Siderotic granules stain bright blue. Siderotic granules stain bright blue.

Safety Data Sheet - Peripheral Blood and Bone Marrow Stain Kit (Solution I)

Safety Data Sheet - Peripheral Blood and Bone Marrow Stain Kit (Solution II)

Safety Data Sheet - Peripheral Blood and Bone Marrow Stain Kit (Solution III)