Peroxidase-Conjugated Goat IgG Fraction to Mouse Immunoglobu

Analysis Note: The total protein is measured using the Biuret procedure with bovine albumin as the standard. To ensure that the product titer falls within the required range, antibody titer is standardized by microtiter plate ELISA with mouse gamma globulin. The product is tested for purity and specificity at final concentration by immunoelectrophoresis. The antibody is predominantly goat IgG; no trace of albumin is detected. Preparation Method: Antibody and highly purified HRP (Rz >3.0) are conjugated under defined conditions to obtain optimally labeled product. Conjugated protein is purified by salt fractionation. The product is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, adjusted to standard titer, filtered through a 0.22 μm filter, vialed and lyophilized. No preservative has been added. Applications: Peroxidase conjugated Goat IgG fraction to mouse immunoglobulins(IgG, IgA, IgM) is use as a reagent in enzyme immunoassays (EIA), cell and tissue staining (for light microscopy), cell and tissue labeling (for electron microscopy), and blot immunostaining. (Note: F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors. Affinity purified antibodies or their fragments are recommended to avoid non-specific binding from inherent antibodies of host animals). Detection of antibodies in sera of T. gondii-infected mice was done by ELISA using peroxidase-conjugated anti-mouse polyvalent immunoglobulin antibody as the secondary. Product Description: Product is the lyophilized powder of horseradish peroxidase (HRP)-conjugated goat IgG fraction to mouse immunoglobulins (IgG,IgA,IgM) and buffer salts. Recommended Use: Reconstitution: Reconstitute product with 2.0 mL of deionized or distilled water. Gentle swirling may be used to speed rehydration. Do not add sodium azide. Avoid vigorous shaking of the reconstituted material.