Description: Product is horseradish peroxidase (HRP)-conjugated rabbit affinity purified antibody to goat IgG (whole molecule) and buffer salts. Antibody and highly purified HRP (Rz>3.0) are conjugated under defined conditions to obtain optimally labeled product. Conjugated protein is purified by salt fractionation. The product is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, with 1% BSA, adjusted to standard titer, filtered through a 0.22 µm filter, vialed, and lyophilized. No preservative is added. Uses: This product is suitable for use as a reagent in enzyme immunoassays (EIA), cell and tissue staining (for light microscopy), cell and tissue labeling (for electron microscopy), and blot immunostaining. Use of affinity purified antibodies is recommended to avoid non-specific binding from the inherent antibodies of host animals. (Note: F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors. See Related Product Ordering Information for these products.) Suggested Working Dilutions: 1:200 - 1:800 for Tissue Staining 1:500 - 1:2,000 for Immunoblotting 1:1,000 - 4,000 for Elisa It is recommended that each lab obtain their own optimal working dilution for each assay. Reconstitution: Reconstitute product with 2.0 ml of deionized or distilled water. Gentle swirling may be used to speed rehydration. Do not add sodium azide. Avoid vigorous shaking of the reconstituted material. Storage: Store product at 2-8°C. Store reconstituted material no longer than 4 weeks at 2-8°C. For long term storage, aliquot and store with 40% glycerol (v/v) at -20°C.
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- UPC:
- 41116127
- Condition:
- New
- Availability:
- 3 Days
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 855363
- Temperature Control Device:
- Yes
