PEROXIDE ASSAY KIT (C15-1884-321)

General description

Peroxide (e.g. hydrogen peroxide H2O2), a reactive oxygen species produced through the metabolism of molecular oxygen, serves as both an intracellular signaling messenger and a source of oxidative stress. Hydrogen peroxide is generated in cells via multiple mechanisms such as the NOX-mediated ROS production by neutrophils and macrophages (respiratory burst) or by the dismutase of superoxide anions produced as a result of electron leak during mitochondrial respiration. Abnormal hydrogen peroxide production contributes to oxidative cell damage and the progression of diseases such as asthma, atherosclerosis, osteoporosis, and neurodegeneration.

Application

Peroxide Assay Kit has been used to measure hydrogen peroxide level in rat tissue homogenates.

Features and Benefits

Compatible with high-throughput handling systems.

Suitability

Suitable for the quantitative determination of peroxide concentration without any pretreatment in a variety of biological samples, such as serum, citrate-plasma, urine, cell lysates, and culture medium.

Principle

The Peroxide Assay Kit provides a simple and high-throughput adaptable assay for quantitative determination of peroxide concentration without any pretreatment in a variety of biological samples, such as serum, citrate-plasma, urine, cell lysates, and culture medium. This assay utilizes the chromogenic Fe³⁺ −xylenol orange reaction, in which a purple complex is formed when Fe²⁺ is oxidized to Fe³⁺ by peroxides present in the sample, generating a colorimetric (585 nm) result, proportional to the level of peroxide present. The optimized formulation reduces interference by substances in the raw samples. A concentration of 1 μM H₂O₂ equals 34 ng/mL or 34 ppb. The kit has a detection range of 0.2–30 μM (7–1,020 ng/mL) H₂O₂ in a 96 well format.