General description
Note: 1 set = 2 x 1 ml.
Synthesized from isoamyl nitrite and hydrogen peroxide. Peroxynitrite (ONOO–) is a strong biological oxidizing agent that reacts with DNA, membrane phospholipids, sulfhydryl groups, and tyrosine. It has been implicated in a variety of patho-physiological conditions including acute respiratory distress syndrome (ARDS), arthritis, atherosclerosis, inflammatory bowel disease, ischemia-reperfusion, N-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-mediated neurotoxicity, and septic shock. This product decomposes or isomerizes at pH 8 or less; the half-life at pH 7.5 is ~1.5 s. ε302 nm = 1670M-1cm-1 in 0.1 MNaOH.
Synthesized from isoamyl nitrite and hydrogen peroxide. Peroxynitrite (ONOO–) is a cell-permeable strong biological oxidizing agent that reacts with DNA, membrane phospholipids, sulfhydryl groups, and tyrosine. Has been implicated in a variety of pathophysiological conditions including acute respiratory distress syndrome (ARDS), arthritis, atherosclerosis, inflammatory bowel disease, ischemia-reperfusion, N-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-mediated neurotoxicity, and septic shock.
Biochem/physiol Actions
Cell permeable: yes
Primary Target
Oxidizing agent that reacts with DNA, membrane phospholipids, sulfhydryl groups, and tyrosine
Product does not compete with ATP.
Reversible: no
Packaging
Packaged under inert gas
Warning
Toxicity: Oxidizer (K)
Physical form
Supplied in 4.7% NaOH at 160-200 mM.
Preparation Note
Peroxynitrite is soluble in aqueous buffers that have been pre-purged with an inert gas and pre-cooled to 4°C.
Reconstitution
Protect from heat, light, metal ions, and air. Peroxynitrite is unstable; thaw rapidly, dispense into aliquots, purge tubes with an inert gas, and freeze -70°C. Activity decreases ~2% /day at -20°C.
Other Notes
Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
Marla, S.S., et al. 1997. Proc. Natl. Acad. Sci. USA94, 14243.
Sakuma, S., et al. 1997. Biochem. Biophys. Res. Commun. 230, 476.
Lymar, S.V., et al. 1996. Biochemistry 35, 7855.
Uppu, R.M., and Pryor, W.A. 1996. Methods Enzymol. 269, 322.
Ischiropoulos, H., and al-Medhi, A.B. 1995. FEBS Lett. 364, 279.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Quality Level: 100. form: liquid. manufacturer/tradename: Calbiochem®. . storage condition: OK to freeze, avoid repeated freeze/thaw cycles, protect from light. color: yellow. shipped in: wet ice. storage temp.: −. 70°C. Pictograms: GHS05,GHS08. Signal Word: Danger. Hazard Statements: H290 - H314 - H341 - H351. Precautionary Statements: P202 - P234 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338. Hazard Classifications: Carc. 2 - Eye Dam. 1 - Met. Corr. 1 - Muta. 2 - Skin Corr. 1B. Storage Class Code: 5.1B - Oxidizing hazardous materials. WGK: WGK 2. Flash Point(F): Not applicable. Flash Point(C): Not applicable.- UPC:
- 51171521
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 516620-1SET