Pertussis Toxin, Bordetella pertussis - CAS 70323-44-3 - Calbiochem

General description

A protein endotoxin that catalyzes ADP-ribosylation of guanine nucleotide-binding regulatory proteins G_i, G_o, and G_T. Affects glucose homeostasis, immune cell function, and the induction of leukocytosis. Used in the study of adenylate cyclase regulation and the role of G_i proteins. Holotoxin activity is determined in CHO cell assay. Note: this product is not activated. If used with an intact cell system, the cells will activate the toxin. If used in a cell-free system, activation may be achieved by the methods of either Kaslow, H.R., and Burns, D.L. 1992. and Moss, J. 1983.

NOTE: This product is not activated, hence for use in cell-free systems, activation is required.

Obtain a uniform suspension before use. A protein endotoxin that catalyzes ADP-ribosylation of guanine nucleotide-binding regulatory proteins G_i, G_o, and G_t. Used in the study of adenylate cyclase regulation and the role of G_i proteins. Holotoxin activity is determined in a CHO cell assay.

This Pertussis toxin, CAS 70323-44-3, catalyzes ADP-ribosylation of guanine nucleotide-binding regulatory proteins Gi, Go, and Gt.

Biochem/physiol Actions

Primary Target
Guanine nucleotide-binding regulatory proteins Gi, Go, and Gt

Product does not compete with ATP.

Warning

Toxicity: Toxic (F)

Physical form

Lyophilized from 50 mM NaCl, 10 mM sodium phosphate buffer, pH 7.0.

Reconstitution

Following reconstitution, refrigerate (4°C). For long-term storage in solution, reconstitute with a high ionic strength buffer, such as 100 mM sodium phosphate, 500 mM sodium chloride, pH 7.0. Stock solutions are stable for at least 6 months for use in CHO cell assays and for 1-2 months for use in non-cellular systems.

Reconstitute with 500 µl H₂O. Obtain a uniform suspension before each use by scraping the walls of the tube and gently pipetting the solution to mix.

Analysis Note

Five distinct bands by SDS-PAGE

Other Notes

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Joshi, S.A., et al. 1998. FEBS Lett. 441, 67.
Fryer, M.W. 1992. Neurosci. Lett.146, 84.
Gierschik, P. 1992. Curr. Topics Microbiol. Immunol.175, 69.
Harada, J., et al. 1992. Eur. J. Pharmacol.227, 301.
Hausman, S.Z., and Burns, D.L. 1992. J. Biol. Chem.267, 13735.
Kaslow, H.R., and Burns, D.L., 1992. FASEB J.6, 2684.
Zhu, J., et al. 1992. J. Pharmacol. Exp. Therap.263, 1479.
Hewlett, E.L., et al. 1983. Infect. Immun.40, 1198.
Moss, J., et al. 1983. J. Biol. Chem.258, 11879.

Legal Information

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