PF97387 (C15-1314-359)

General description

Homozygous: Yes

Cell Line Description

This cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.

DNA Profile

A: 2902
Cw: 1601
B: 44031
HLA DRB1: 04
HLA DQA1: 3
HLA DQB1: 03
HLA DPA1: 1
HLA DPB1: 0401 0402

Serological Profile

HLA A: 29
HLA A ief: 29.2
HLA B: 44
HLA B ief: 44.1
Bw4/bw6: 4
HLA Dw: 4
HLA DR: 4
HLA DR53: 53
HLA DQ: 7
HLA DP: 4

Culture Medium

RPMI 1640 (R0883) + 2mM Glutamine (G7513) + 20% Foetal Bovine Serum (FBS) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.

Subculture Routine

Maintain cultures between 3x10⁵ - 2x10⁶ cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Disclaimer

This cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.

biological source: human blood. ethnicity: French. gender: male. growth mode: Suspension. karyotype: Not specified. morphology: Lymphoblastoid. technique(s): cell culture | mammalian: suitable. shipped in: dry ice.