Phaseolus vulgaris lectin (PHA-E) is isolated from red kidney beans and purified by affinity chromatography and ion-exchange chromatography. It is a tetrameric protein with a molecular weight of 128 kDa. The lectin recognizes and binds specifically to terminal galactose, N-acetylglucosamine and mannose residues of complex glycans on mammalian glycoproteins. Because of its strong erythrocyte agglutinating activity, it is also known as Erythroagglutinin (PHA-E). PHA-E is not blood group specific. Less than 5 μg/ml agglutinates human erythrocytes of type O and agglutination can be inhibited by certain oligosaccharides. PHA-E lectin is supplied as a white lyophilized powder from a buffer containing from 50 mM NH4HCO3. The purity of the lectin in determined by SDS-PAGE, which generate one single band at 34 kDa. ● Ultrapure quality ● Sugar specificity: complex oligosaccharides ● Strong erythroagglutinin activity ● Lyophilized powderErythrocyte agglutination studies;Model system of how proteins recognize carbohydrates
Related Documents: https://ald-pub-files.oss-cn-shanghai.aliyuncs.com/aladdinsci/pdp/sds/1/P140098-SCI_5147b1cd0ca967fbf32adf402a3be9b3.pdf
- UPC:
- 41131617
- Condition:
- New
- Availability:
- 4-8 weeks
- Weight:
- 1.06 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- P140098-1mg
- Product Size:
- 1mg
