Phaseolus vulgaris lectin P (PHA-P), native isomer composition (C09-1017-486)

Features Ultrapure qualityAgglutinates leucocytes and erythrocytesMixture of L and E subunitsSugar specificity: complex oligosaccharidesLyophilized powder Product descriptionPhaseolus vulgaris lectin P or Phytohemagglutinin (PHA-P)is isolated from red kidney beans and purified by affinitychromatography. PHA-P is a mixture of isolectins containingL (leucoagglutinin) and E (erythroagglutinin) subunits incompositions L1E3, L2E2, L3E1, E4.Thus PHA-P is the form of PHA before separation oferythroagglutinin and leucoagglutinin. PHA-P has amolecular weight of 128 kDa and specificity for complexoligosaccharides . PHA-P is not blood group specific and agglutinates human erythrocytes blood group 0 with alectin concentration of ≤4 μg/ml.PHA-P lectin is supplied as a white to light-pinkessentially salt-free lyophilized powder from 50 mM NH4HCO3,which evaporates during the lyophilization procedure. Thepurity of the lectin is determined by SDS-PAGE generatingone single band.ApplicationsLateral flow erythrocyte separationTypical subunit compositionTotal L subunit composition: 60-65%Total E subunit composition: 35-40%L4 25-35%L3E1 20-30%L2E2 10-20%L1E3 17-27%E4 1-11%Directions for useThe lectin may be reconstituted in a suitable buffer for yourapplication.Shipping and storageThe product is shipped at -20°C, however for over-the-daytransport it may be shipped at ambient temperature. Thelyophilized powder is stable for more than five years fromproduction date when stored below -20°C. After reconstitutionwith PBS, pH 7.4, the solution may be stored frozen in workingaliquots for up to 12 months. Related Document: https://ald-pub-files.oss-cn-shanghai.aliyuncs.com/aladdinsci/pdp/sds/1/P419332-SCI_206ef9fbf45d1760de3cda1e0dd66895.pdf.