Phenol Red Dextrose Broth, 2.5 kg

Phenol Red Broth Medium is formulated as per Vera (2) and is recommended to determine the fermentation reaction of carbohydrates for the differentiation of microorganisms (3, 4, 5). Phenol Red Broth Medium with various carbohydrates serves as a differential medium by aiding in differentiation of various species and genera by their ability to ferment the specific carbohydrate, with the production of acid or acid and gas (6). Phenol Red Dextrose Broth is used to study dextrose fermentation in various bacteria. Proteose peptone and beef extract serve as sources for carbon and nitrogen. Sodium chloride is the osmotic stabilizer. Phenol red is the pH indicator, which turns yellow at acidic pH i.e. on fermentation of dextrose. Gas formation is seen in Durhams tubes. All of the Enterobacteriaceae grow well in this medium. In addition to producing a pH color shift, the production of mixed acids, notably butyric acids, often results in a pungent, foul odor from the culture medium (1). Storage and Shelf-life: Store below 30°C in tightly closed container and the prepared medium at 2-8°C. Use before expiry date on the label. References: 1. Koneman E. W., Allen S. D., Janda W.M., Schreckenberger P.C., Winn W.C. Jr., 1992, Colour Atlas and Textbook of Diagnostic Microbiology, 4th Ed., J. B. Lippinccott Company 2. Vera H. D., 1950, Am. J. Public Health, 40, 1267 3. MacFaddin J. F., 1985, Media for Isolation-Cultivation-Identification -Maintenanceof Medical Bacteria, Vol. I, Williams and Wilkins, Baltimore. 4. Finegold S. M. and Baron E. J., 1986, Bailey and Scotts Diagnostic Microbiology, 7th Ed., The C.V. Mosby Co., St. Louis. 5. Ewing W. H., 1986, Edwards and Ewings Identification of Enterobacteriaceae, 4th ed.,Elsevier Science Publishing Co.,Inc., New York. 6. MacFaddin J. F., 2000, Biochemical tests for Identification of Medical Bacteria, 3rd edi., Lippincott, Williams and Wilkins, Baltimore.