General description
Phosphate-ON phosphoramidite is a chemical phosphorylation reagent that can be employed to introduce a phosphate group at the 5′ terminus of an oligonucleotide. Chemical phosphorylation is a cost-effective alternative to enzymatic phosphorylation methods, allowing the introduction of terminal phosphate groups in high yields, at any desired scale. We offer Phosphate-ON phosphoramidite in powder form, enabling the dissolution of the reagent in acetonitrile to be easily monitored.
Application
The application of Phosphate-ON Phosphoramidite is fully compatible with standard cleavage and deprotection procedures at the end of oligonucleotide synthesis. Phosphate-ON phosphoramidite comprises a thymidine nucleoside, which is removed from the oligonucleotide during the cleavage and deprotection process.
Other Notes
The key features of Phosphate-ON Phosphoramidite are as follows:
- Powder format enables dissolution to be easily monitored and facilitatesproduct handling
- Fully compatible with standard phosphoramidite reagents and synthesisconditions
- Compatible with dG(dmf) fast deprotection chemistry: the oligonucleotidecan be cleaved from the support and deprotected in concentratedammonia at 55 °C in only 2 hours
- Comprises a DMT-group for trityl monitoring
- Dissolves easily in acetonitrile to a 0.1 M solution
- High coupling efficiency of ≥98.0% under standard DNA phosphoramiditecoupling conditions
- Cleavage of both phosphate protective groups through a β-eliminationprocess
Legal Information
This product is covered under US Patent Number 7,276,598
Shipping Information:
Hazmat Fee: $65.00 + applicable shipping charges are added after the order is placed.
More Information: https://cenmed.com/shipping-returns
- UPC:
- 41116134
- Condition:
- New
- Availability:
- 3-5 Days
- Weight:
- 1.00 Ounces
- HazmatClass:
- Yes
- MPN:
- M061040-1G
