PHOSPHODETECT(TM) ANTI-CHK1 (PS 1PC X 50UL

General description

Immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~56 kDa Chk1 protein when phosphorylated at Ser³¹⁷.

Recognizes the ~56 kDa Chk1 protein phosphorylated at Ser³¹⁷ in UV-treated COS cells.

This PhosphoDetect Anti-Chk1 (pSer³¹⁷) Rabbit pAb is validated for use in Immunoblotting, Immunocytochemistry, Paraffin Sections for the detection of Chk1 (pSer³¹⁷).

Immunogen

Human

a synthetic phosphopeptide corresponding to amino acids surrounding the Ser³¹⁷ phosphorylation site of human Chk1

Application

Immunoblotting (1:1000)
Immunocytochemistry (1:500, fluorescence)
Paraffin Sections (1:50)

Warning

Toxicity: Standard Handling (A)

Physical form

In 150 mM NaCl, 10 mM HEPES, 100 µg/ml BSA, 50% glycerol, pH 7.5.

Reconstitution

Do not aliquot.

Analysis Note

Positive Control
Lysates from COS cells treated with UV

Other Notes

Does not recognize unphosphorylated Chk1. Antibody should be titrated for optimal results in individual systems.

Zhao, H. and Piwnica-Worms, H. 2001. Mol. Cell. Biol.21, 4129.
Shieh, S.Y., et al. 2000. Genes Dev.14, 289.
Martinho, R.G., et al. 1998. EMBO J.17, 7239.
Zeng, Y., et al. 1998. Nature395, 507.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany