General description
Rabbit polyclonal antibody adsorbed against non-phosphorylation site, followed by immunoaffinity chromatography. Recognizes the ~170 kDa EGFR protein phosphorylated at Tyr1068.
Recognizes the ~185 kDa EGFR protein phosphorylated at Tyr1068. Note: 1 T = 1 test. Sufficient for 10 Western miniblots.
This PhosphoDetect
Immunogen
a synthetic phosphopeptide corresponding to amino acids surrounding the Tyr¹⁰⁶⁸ phosphorylation site of human EGFR
Warning
Toxicity: Standard Handling (A)
Physical form
In Dulbecco′s PBS, 1 mg/ml BSA, 50% glycerol, pH 7.4.
Other Notes
Jo, M., et al. 2000. J. Biol. Chem.275, 8806.
Maudsley, S., et al. 2000. J. Biol. Chem.275, 9572.
Sieg, D.J. et al. 2000. Nat. Cell. Biol.2, 249.
Barbier, A.J. et al. 1999. J. Biol. Chem.274, 14067.
Hashimoto, A. et al. 1999. J. Biol. Chem.274, 20139
Poppleton, H.M. et al. 1999. Arch. Biochem. Biophys.363, 227.
Batzer, A.G. et al. 1994. Mol. Cell. Biol.14, 5192.
When diluted 1:1000 there is sufficient antibody to perform 10 miniblots (10 T) at 10 ml per blot. Variables associated with assay conditions will dictate the proper working dilution.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
biological source: rabbit. Quality Level: 100. antibody product type: primary antibodies. clone: polyclonal. form: liquid. contains: ≤. 0.1% sodium azide as preservative. species reactivity: human, mouse, chicken, rat. manufacturer/tradename: Calbiochem®. . storage condition: OK to freeze, avoid repeated freeze/thaw cycles. isotype: IgG. shipped in: wet ice. storage temp.: −. 20°C. target post-translational modification: phosphorylation (pTyr1068). Gene Information: chicken ... Egfr(396494)human ... EGFR(1956)
mouse ... Egfr(13649)
rat ... Egfr(24329). Storage Class Code: 10 - Combustible liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.
- UPC:
- 51124200
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 324867-10T
