General description

Rabbit polyclonal antibody adsorbed against non-phosphorylation site, followed by immunoaffinity chromatography. Recognizes the ~170 kDa EGFR protein phosphorylated at Tyr¹⁰⁶⁸.

Recognizes the ~185 kDa EGFR protein phosphorylated at Tyr¹⁰⁶⁸. Note: 1 T = 1 test. Sufficient for 10 Western miniblots.

This PhosphoDetect Anti-EGFR (pTyr¹⁰⁶⁸) Rabbit pAb is validated for use in Immunoblotting for the detection of EGFR (pTyr¹⁰⁶⁸).

Immunogen

a synthetic phosphopeptide corresponding to amino acids surrounding the Tyr¹⁰⁶⁸ phosphorylation site of human EGFR

Warning

Toxicity: Standard Handling (A)

Physical form

In Dulbecco′s PBS, 1 mg/ml BSA, 50% glycerol, pH 7.4.

Other Notes

Jo, M., et al. 2000. J. Biol. Chem.275, 8806.
Maudsley, S., et al. 2000. J. Biol. Chem.275, 9572.
Sieg, D.J. et al. 2000. Nat. Cell. Biol.2, 249.
Barbier, A.J. et al. 1999. J. Biol. Chem.274, 14067.
Hashimoto, A. et al. 1999. J. Biol. Chem.274, 20139
Poppleton, H.M. et al. 1999. Arch. Biochem. Biophys.363, 227.
Batzer, A.G. et al. 1994. Mol. Cell. Biol.14, 5192.

When diluted 1:1000 there is sufficient antibody to perform 10 miniblots (10 T) at 10 ml per blot. Variables associated with assay conditions will dictate the proper working dilution.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

biological source: rabbit. Quality Level: 100. antibody product type: primary antibodies. clone: polyclonal. form: liquid. contains: ≤. 0.1% sodium azide as preservative. species reactivity: human, mouse, chicken, rat. manufacturer/tradename: Calbiochem^®.. storage condition: OK to freeze, avoid repeated freeze/thaw cycles. isotype: IgG. shipped in: wet ice. storage temp.: −. 20Â°C. target post-translational modification: phosphorylation (pTyr1068). Gene Information: chicken ... Egfr(396494)
human ... EGFR(1956)
mouse ... Egfr(13649)
rat ... Egfr(24329). Storage Class Code: 10 - Combustible liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.