PhosphoDetect Anti-GSK3beta (pSer⁹) Mouse mAb (2D3) (C15-1303-039)

General description

Mouse monoclonal antibody purified by thiophilic adsorption and size exclusion chromatography. Recognizes the ~46 kDa GSK-3β phosphorylated at Ser⁹. Supplied with a control lysate derived from 2 x 10⁶ EGF-treated HepG2 cells.

Recognizes the ~46 kDa GSK-3β phosphorylated at Ser⁹. Supplied with a control lysate derived from 2 x 10⁶ EGF-treated HepG2 cells.

This PhosphoDetect Anti-GSK3β (pSer⁹) Mouse mAb (2D3) is validated for use in ELISA, Immunoblotting for the detection of GSK3β (pSer⁹).

Immunogen

Human

a synthetic phosphopeptide corresponding to amino acids surrounding the Ser⁹ phosphorylation site of human glycogen synthase kinase 3β

Application

ELISA (0.05 µg/ml)

Immunoblotting (0.5 µg/ml)

Warning

Toxicity: Standard Handling (A)

Physical form

100 µg antibody in PBS, PEG, sucrose, 50% glycerol and 200 µl lyophilized control lysate.

Reconstitution

Reconstitute the positive control lysate with 200 µl H₂O. Following complete solubilization, add 200 µl SDS-PAGE sample buffer and incubate at 90°C for 5 min.

Analysis Note

Positive Control
Pervanadate-treated HepG2 (included)

Other Notes

For immunoblotting with the positive control, use 10 µl (chemiluminescence) or 20 µl (colorimetric) per mini-gel lane. Variables associated with assay conditions will dictate the proper working dilution.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

biological source: mouse. Quality Level: 100. antibody form: purified antibody. antibody product type: primary antibodies. clone: 2D3, monoclonal. form: liquid. contains: ≤. 0.1% sodium azide as preservative (antibody only). species reactivity: mouse, human. manufacturer/tradename: Calbiochem^®. . storage condition: OK to freeze. isotype: IgG1. shipped in: wet ice. storage temp.: −. 20°C. target post-translational modification: phosphorylation (pSer9). Gene Information: human ... GSK3B(2932)
mouse ... Gsk3B(56637). Storage Class Code: 11 - Combustible Solids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.