PHOSPHODETECT(TM) ANTI-PDH-E1ALPHA 1PC X 50UG (C15-1319-063)

General description

Immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~44 kDa PDH-E1α protein phosphorylated at Ser³⁰⁰.

Recognizes the ~44 kDa PDH-E1α protein phosphorylated at Ser³⁰⁰ in HEK293 cells.

This PhosphoDetect Anti-PDH-E1α (pSer³⁰⁰) Rabbit pAb is validated for use in Immunoblotting, Immunocytochemistry for the detection of PDH-E1α (pSer³⁰⁰).

Immunogen

Human

a synthetic phosphopeptide corresponding to amino acids surrounding the Ser³⁰⁰ phosphorylation site of human PDH-E1α

Application

Immunoblotting (0.5 μg/ml)
Immunocytochemistry (1 μg/ml)

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Physical form

In 150 mM NaCl, 100 mM Tris-Glycine, pH 7.4.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Analysis Note

Negative Control
HEK293 cells treated with dichloroacetate

Positive Control
HEK293 cells

Other Notes

Due to a high degree of sequence identity, this antibody is also expected to cross-react with mouse, rat, bovine, Zebra fish, and Xenopus phospho-PDH-E1α Antibody should be titrated for optimal results in individual systems.

Rardin M.J., et. al. 2009. Anal. Biochem.2, 157.
Seifert, F., et al. 2007. Biochemistry 21, 6277.
Lee, J., et al. 2007. Mol. Cell Prot. 4, 669.
Patel, M.S. and Korotchkina, L.G. 2006 Biochem. Soc. Trans.34, 217.
Korotchkina, L.G., et al. 2001. J. Biol. Chem. 40, 37223.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

biological source: rabbit. Quality Level: 200. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. form: liquid. contains: ≤. 0.1% sodium azide as preservative. species reactivity: human. manufacturer/tradename: Calbiochem^®. . storage condition: OK to freeze, avoid repeated freeze/thaw cycles. shipped in: wet ice. storage temp.: −. 20°C. target post-translational modification: phosphorylation (pSer300). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.

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