PHOSPHODETECT(TM) ANTI-PHOSPHOT 1PC X 100UG (C15-1304-866)

General description

Peroxidase conjugate of Anti-Phosphotyrosine Mouse mAb (PY20), Cat. No. 525295. Recognizes a broad spectrum of tyrosine-phosphorylated proteins in their native and denatured states in crude extracts.

Purified mouse monoclonal antibody conjugated to horseradish peroxidase. Recognizes a broad spectrum of tyrosine-phosphorylated proteins.

This PhosphoDetect Anti-Phosphotyrosine Mouse mAb (PY20) Peroxidase Conjugate is validated for use in ELISA, Immunoblotting, Immunohistochemistry for the detection of Phosphotyrosine.

Immunogen

KLH conjugated to phosphotyrosine, glycine, and alanine

Application

ELISA (1:2000)

Immunoblotting (1:500-1:2000)

Immunohistochemistry (1:500)

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Physical form

In PBS, 50% glycerol, 1% BSA, pH 7.4.

Other Notes

Glenney, J.R., et al. 1988. J. Immunol. Methods109, 277.

Specific for tyrosine phosphorylated proteins in both the native and denatured states. Binding is inhibited by phosphotyrosine; does not react with threonine or serine phosphorylated residues on proteins. Do not use milk as a blocking agent with this antibody. Milk contains many phosphotyrosine containing proteins and will adsorb the antibody. Use a buffer with <150 mM NaCl and divalent cations not much >1 mM because this antibody′s binding is inhibited by high salt concentrations and divalent cations. Variables associated with assay conditions will dictate the proper working dilution.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

biological source: mouse. Quality Level: 100. antibody form: purified antibody. antibody product type: primary antibodies. clone: PY20, monoclonal. form: liquid. contains: 0.1% Proclin as preservative. species reactivity (predicted by homology): all. manufacturer/tradename: Calbiochem^®. . storage condition: OK to freeze. isotype: IgG2b. shipped in: wet ice. storage temp.: 2-8°C. target post-translational modification: phosphorylation (pTyr). Gene Information: human ... PID1(55022). Storage Class Code: 10 - Combustible liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.