General description
Rabbit polyclonal antibody adsorbed against non-phosphopeptide corresponding to the immunogen phosphorylation site, followed by immunoaffinity chromatography. Recognizes the ~49 kDa JNK1/SAPK1 protein and the ~55 kDa JNK2/SAPK2 protein phosphorylated at Thr183 and Tyr185.
Recognizes the ~49 kDa SAPK1 protein and the ~55 kDa SAPK2 protein phosphorylated at Thr183 and Tyr185.
This PhosphoDetect
Immunogen
Human
a synthetic phosphopeptide corresponding to amino acids surrounding the Thr¹⁸³ and Tyr¹⁸⁵ phosphorylation sites of human SAPK/JNK
Application
Immunoblotting (1:1000)
Immunohistochemistry (see comments)
Warning
Toxicity: Standard Handling (A)
Physical form
In Dulbecco′s PBS (without Mg2+ and Ca2+), 50% glycerol, 1 mg/ml BSA, pH 7.3.
Reconstitution
Following initial thaw, aliquot and freeze (-70°C).
Other Notes
Fanger, G.R., et al. 1999. Histol. Histopathol.14, 887.
Gabai, V.L., et al. 1998. FEBS Lett.438, 1.
Specific for the dually phosphorylated form of JNK/SAPK containing a phosphate on threonine 183 and tyrosine 185. This antibody has also been reported to work for immunohistochemistry. Variables associated with assay conditions will dictate the proper working dilution.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
biological source: rabbit. Quality Level: 100. antibody product type: primary antibodies. clone: polyclonal. form: liquid. contains: ≤. 0.1% sodium azide as preservative. species reactivity: Drosophila, chicken, Caenorhabditis elegans, human, mouse, rat. manufacturer/tradename: Calbiochem®. . storage condition: OK to freeze, avoid repeated freeze/thaw cycles. isotype: IgG. shipped in: wet ice. storage temp.: −. 70°C. target post-translational modification: phosphorylation (pThr183/pTyr185). Gene Information: human ... MAPK8(5599). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.- UPC:
- 51313206
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 559309-10T