PHOSPHODETECT(TM) ANTI-SMAD3 (P 1PC X 50UL

General description

Purified rabbit monoclonal antibody generated by immunizing rabbits with the specified immunogen and fusing splenocytes with an immortal cell line. Recognizes the ~52 kDa Smad3 protein phosphorylated at Ser^423/425.

Recognizes the ~52 kDa Smad3 protein phosphorylated at Ser^423/425 in HT-1080 cells treated with TGF-β1.

This PhosphoDetect Anti-Smad3 (pSer^423/425) Rabbit mAb (C25A9) is validated for use in Immunoblotting, Immunoprecipitation for the detection of Smad3 (pSer^423/425).

Immunogen

a synthetic phosphopeptide corresponding to amino acids surrounding the Ser ^423/425 phosphorylation sites of Smad3, coupled to KLH.

Application

Immunoblotting (1:1000)
Immunoprecipitation (1:50)

Warning

Toxicity: Standard Handling (A)

Physical form

In 150 mM NaCl, 10 mM HEPES, 100 µg/ml BSA, 50% glycerol pH 7.5.

Other Notes

Attisano, L., et al. 2002. Science 296, 1646.
Moustakas, A., et al. 2001. J. Cell Sci. 114, 4359.
Wrana, J. 2000. Science 23, 1.

Does not cross-react with other Smad proteins. For immunoblotting incubate the membrane with antibody diluted with TBS containing 5% BSA, 0.1% Tween^®-20 detergent overnight at 4°C with gentle shaking. Please note that this is a rabbit monoclonal antibody and requires the use of an anti-rabbit secondary antibody. The immunogen sequence is 100% conserved in mouse, rat, monkey, bovine, xenopus, and zebrafish Smad3, but cross-reactivity has not been tested. Antibody should be titrated for optimal results in individual systems.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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