PHOSPHODETECT(TM) ANTI-SYK (PTYR525/526) RABBIT PAB

General description

Protein A and immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~72 kDa Syk protein phosphorylated on Tyr^525/526.

Recognizes the ~72 kDa Syk protein phosphorylated at Tyr^525/526 in human Syk or Tyr^519/520 in mouse Syk in 293T cells expressing wild-type Syk and CD8.

This PhosphoDetect Anti-Syk (pTyr^525/526) Rabbit pAb is validated for use in ELISA, Immunoblotting for the detection of Syk (pTyr^525/526).

Immunogen

Human

a synthetic phosphopeptide corresponding to amino acids surrounding the Tyr^525/526 phosphorylation sites of human Syk

Application

ELISA (1:1000)
Immunoblotting (1:1000)

Warning

Toxicity: Standard Handling (A)

Physical form

In 150 mM NaCl, 10 mM sodium HEPES, 100 µg/ml BSA, 50% glycerol, pH 7.5.

Reconstitution

Do not aliquot.

Analysis Note

Positive Control
293T cells transfected with wild-type Syk and CD8

Other Notes

Coopman, P.J., et al. 2000. Nature406, 742.
Turner, M., et al. 2000. Immunol. Today21, 148.
Zang, J., et al. 2000. J. Biol. Chem.275, 35442.
Chu, D.H., et. al. 1998. Immunol. Rev.165, 167.
Cheng, A.M. and Chan, A.C. 1997. Curr. Opin. Immunol.9, 528.
Kurosaki, T. 1997. Curr. Opin. Immunol.9, 309.

Tyr^525/526 represent the phosphorylation sites in human Syk, while Tyr^519/520 are the equivalent phosphorylation sites in mouse Syk. For immunoblotting the membrane should be incubated with antibody diluted in 5% BSA, 1X TBS, 0.1% Tween^®-20 detergent at 4°C overnight with gentle shaking. Antibody should be titrated for optimal results in individual systems.

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