General description
Full-length, recombinant, human phosphodiesterdase 3A (PDE3A) expressed in S. frugiperda insect cells using a baculovirus expression system. PDE3A is expressed primarily in cardiac and vascular myocytes and platelets. Supplied as a partially purified preparation of catalytically active enzyme useful for inhibitor and activator assays.
Warning
Toxicity: Standard Handling (A)
Unit Definition
One unit is defined as the amount of enzyme that is required to convert 1 pmol cAMP to AMP per min at pH 7.5, 30°C.
Physical form
In 150 mM NaCl, 10 mM Tris, 0.1% NP-40, pH 8.3.
Reconstitution
Following reconstitution, aliquot and freeze (-70°C).
Other Notes
Shakur, Y., et al. 2000. J. Biol. Chem.275, 38749.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
recombinant: expressed in insect cells (S. frugiperda). Quality Level: 100. form: liquid. specific activity: ≥. 0.5 . U/μ. L. manufacturer/tradename: Calbiochem®. . storage condition: OK to freeze, avoid repeated freeze/thaw cycles. shipped in: wet ice. storage temp.: −. 70°C. Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.- UPC:
- 51472808
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 524742-10U
