PHOSPHODIESTERASE 3B HISTA 1PC X 10U (C15-1304-850)

General description

Full-length, recombinant, human phosphodiesterase 3B (accession no. AY459346) fused at the N-terminus to a His•Tag sequence and expressed in S. frugiperda insect cells using a baculovirus expression system. An important component of insulin and cAMP-dependent signaling. Supplied as a partially purified preparation.

Warning

Toxicity: Standard Handling (A)

Unit Definition

One unit is defined as the amount of enzyme that will convert 1 pmole cAMP to AMP per min at pH 7.5, 30°C.

Physical form

In 150 mM NaCl, 10 mM Tris, 0.1% NP-40, pH 8.3.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Other Notes

Gibson, L.C.D., et al. 2006. Eur. J. Pharmacol.538, 39.
Kitamura, T., et al. 1999. Mol. Cell. Biol.19, 6286.
Degerman, E., et al. 1997. J. Biol. Chem.272, 6823.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany

form: liquid. Quality Level: 100. specific activity: ≥. 0.5 . U/μ. L. manufacturer/tradename: Calbiochem^®. . storage condition: OK to freeze. shipped in: wet ice. storage temp.: −. 70°C. Storage Class Code: 10 - Combustible liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.