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PHOSPHOENOLPYRUVATE CARBOXYLASE FROM MICROBIAL SOURCE

Catalog No.
C005B-089671
Mfr. No.
C1744-1KU
Mfr. Name
Sigma-Aldrich
Qty/UOM
1000
UOM
UN
Price: $580.65
List Price: $645.16

Application Phosphoenolpyruvate carboxylase (PEPC) is an enzyme useful for enzymatic determination of carbon dioxide when coupled with malate dehydrogenase in clinical analysis. PEPC is also used to study carbon assimilation, post-translational

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Application

Phosphoenolpyruvate carboxylase (PEPC) is an enzyme useful for enzymatic determination of carbon dioxide when coupled with malate dehydrogenase in clinical analysis. PEPC is also used to study carbon assimilation, post-translational regulation and allosteric regulation in various plants .

Biochem/physiol Actions

Phosphoenolpyruvate carboxylase (PEPC) catalyzes the addition of bicarbonate to phosphoenolpyruvate (PEP) to form the four-carbon compound oxaloacetate and inorganic phosphate . In CAM and C4 plants, PEPC catalyzes the photosynthetic assimilation of CO2 into an organic acid. PEPC is activated by glucose-6-phosphate and is inhibited by malate and aspartate. PEPC comprises about 0.5-2% of the soluble protein in alfalfa and soybean nodules .

Unit Definition

One unit causes the oxidation of one micromole of NADH per minute at pH 8.0 at 30°C.

Physical form

White amorphous lyophilized powder containing BSA and sugar alchohols as stabilizers.

UPC:
41142048
Condition:
New
Availability:
3-5 Days
Weight:
1.00 Ounces
HazmatClass:
No
MPN:
C1744-1KU
CAS:
9067-77-0

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