General description
Phosphoglucose Isomerase is an enzyme which possesses diagnostic potential when fractionated by cellulose acetate electrophoresis. This cytosolic enzyme plays an important role during glycolysis and glucogenesis. It acts as an tumor-secreted cytokine and autocrine motility factor. It is used as neurotrophic factor, maturation factor and as serine proteinase inhibitor.
Specificity
Phosphoglucose isomerase (PGI) is specific for G6P (Km = 0.7 mM) and fructose-6-phosphate (F6P).
Application
Isomerization of ketoses to aldoses.
Quality
Contaminants: <0.01% F6P-K, GR, 6-PGDH and PGluM, each, <0.2% β-fructosidase
Physical form
Suspension in 3.2 M ammonium sulfate solution, pH approximately 6
Preparation Note
Storage conditions (working solution): A solution of PGI in 20 mM Tris, pH 8.1, containing 0.2 mg BSA/ml, is stable approx. 1 week at 2 to 8 °C.
Other Notes
For life science research only. Not for use in diagnostic procedures.
- UPC:
- 51172601
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 10128139001