Phospholipid quantification Assay Kit (Colorimetric/Fluorometric)

General description

Phospholipids are a class of lipids, which constitute a major component of cell membranes and lipid bilayers. Phospholipids play important roles in signal transduction. The majority of phospholipids contain one diglyceride or sphingosine, a phosphate group, and one choline.

The Phospholipid quantification assay kit provides a simple and highly sensitive method for measuring choline-containing phospholipids in biological samples. This high-throughput assay can be done within only 30 minutes . In this assay, phospholipids (such as lecithin, lysolecithin, and sphingomyelin) are enzymatically hydrolyzed to release choline, which is determined using choline oxidase and an H₂O₂ specific dye. The readout is either fluorescence or absorbance, allowing maximal flexibility and a large detection range. The linear range is 0.1-0.5 nmol (fluorescence) and 1 to 5 nmol (absorbance). Phosphatidylcholine-containing phospholipids are present in serum at ~0.2-2.5 mM (~50-200 mg/dL).

The detection range of the kit is 0.1-0.5 nmol (fluorescence) and 1 to 5 nmol (absorbance).

Application

• Measuring choline-containing phospholipids in biological samples (For example: Serum and Plasma)
• Enables evaluation of drug effects on phospholipids metabolism

Features and Benefits

Our economical Phospholipid quantification assay kit also saves your time and efforts:

• Highly sensitive, simple and high-throughput assay for measuring choline-containing phospholipids in biological samples
• User friendly: No need to weigh or calculate needed amounts of reagents on your own
• Supportive calculator (Click here to download a calculator excel file):will calculate the reagents needed according to your experimental needs, and analyses your results based on your experimental data!
• Quick instruction bench card - to assure your experimental success
• More experiments in one kit - contains sufficient reagents for 200 tests.
• Suitable for both colorimetric and fluorometric detection
• Detection time: only 30 minutes

Suitability

Suitable for the detection of phospholipids in biological samples