Phthaldialdehyde Reagent (C09-1003-283)

Fluoraldehyde OPA Reagent is a ready-to-use solution of o-phthalaldehyde, a fluorescent derivatization reagent for peptide or amino acid detection and quantitation in HPLC. Fluoraldehyde Reagent Solution contains OPA (o-phthalaldehyde), which reacts with primary amines of amino acids, peptide and proteins to enable fluorescent detection and quantitation. The reagent that can be used as a protein or peptide assay reagent or as a pre- or post-column detection reagent for amino acid analysis (HPLC). Reaction of OPA with proteins and peptides yields linear results over a wide range of concentrations. The Fluoraldehyde Reagent Solution is supplied ready to use and enables fast quantitation of proteins or peptides in solution. Highlights: For pre- or post-column amino acid derivatization for fluorescent detection and quantitation Reacts with all primary amine-containing analytes to yield fluorescent isoindole derivatives Provides an accurate measure of both composition and absolute protein-peptide content Ideal for work with recombinant proteins and synthetic peptides Can be used for fluorescent protein or peptide assay Pre-column derivatization mixtures can be injected into LC without any processing Precolumn derivatization reagent for primary amines and amino acids. The fluorescent derivative can be detected by reverse-phase HPLC. The reaction requires OPA, primary amine and a sulfhydryl. In the presence of excess sulfhydryl, amines can be quantitated. In the presence of excess amine, sulfhydryls can be quantitated. Specification: 0.8mg/mL solution (pH 10) MDL Number: MFCD00003335 Molecular Formula: C8H6O2 Molecular Weight: 134.13 EC Number: 211-402-2 PubChem CID: 4807 Isomeric SMILES: C1=CC=C(C(=C1)C=O)C=O Beilstein Registry Number: 878317 Related Documents: https://ald-pub-files.oss-cn-shanghai.aliyuncs.com/aladdinsci/pdp/sds/1/P128626-SCI_41a41cee9308a8f07abf57dd07814a5c.pdf