PKA Inhibitor 14-22 Amide, Cell-Permeable, Myristoylated - Calbiochem (C15-1303-851)

General description

Heat-stable protein kinase inhibitor (PKI) peptide sequence (14 - 22) that has been myristoylated at the N-terminus, enhancing its cell-permeability. The non-myristoylated version of this peptide is a highly specific inhibitor (K_i = 36 nM) of cAMP-dependent protein kinase.

Heat-stable protein kinase inhibitor (PKI) peptide sequence (14-22) that has been myristoylated at the N-terminus, enhancing its cell-permeability. The non-myristoylated version of this peptide is a highly specific inhibitor (K_i = 36 nM) of cAMP-dependent protein kinase (PKA).

Biochem/physiol Actions

Cell permeable: yes

Primary Target
cAMP-dependent protein kinase

Product does not compete with ATP.

Reversible: no

Target K_i: 36 nMf or cAMP-dependent protein kinase

Warning

Toxicity: Standard Handling (A)

Sequence

Myr-N-Gly-Arg-Thr-Gly-Arg-Arg-Asn-Ala-Ile-NH₂

Physical form

Supplied as a trifluoroacetate salt.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Paman, K., et al. 1998. J. Lipid Res. 39, 1091.
Rimon, G., and Rubin M. 1998. Biochim. Biophys. Acta 1380, 289.
Harris, T.E., et al. 1997. Biochem. Biophys. Res. Commun. 232, 648.
Muniz, M., et al. 1997. Proc. Natl. Acad. Sci. USA 94, 14461.
Zoukhri, D., et al. 1997. Am. J. Physiol. 272, C263.
Eichholtz, T., et al. 1993. J. Biol. Chem.268, 1982.
Ward, N.E. and O’Brian, C.A. 1993. Biochemistry32, 11903.
Walsh, D.A. and Glass, D.B. 1991. Methods Enzymol. 201, 304.
Glass, D.B., et al. 1989. J. Biol. Chem.264, 8802.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Molecular Weight: 1209.49. Empirical Formula: C₅₃H₁₀₀N₂₀O₁₂. Quality Level: 100. Assay: ≥. 95% (HPLC). form: lyophilized. manufacturer/tradename: Calbiochem^®. . storage condition: OK to freeze, desiccated (hygroscopic). solubility: water: 1 . mg/mL. shipped in: ambient. storage temp.: −. 20°C. Storage Class Code: 11 - Combustible Solids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.