General description
Full-length, recombinant, human PKCδ expressed in S. frugiperda insect cells using a baculovirus expression system. PKC δ is classified as a novel PKC isozyme because it does not exhibit calcium dependence and it is activated in vitro by phorbol esters. Suitable for studies of PKCδ and its regulation. PKCδ is a Ca2+-independent and phospholipid-dependent enzyme.
Packaging
Please refer to vial label for lot-specific concentration.
Warning
Toxicity: Standard Handling (A)
Unit Definition
One unit is defined as the amount of enzyme that will transfer 1 nmol of phosphate to PKCε Peptide Substrate (Cat. No. 539562) per min at 30°C, pH 7.5.
Physical form
In 250 mM NaCl, 20 mM HEPES, 5 mM DTT, 2 mM EDTA, 2 mM EGTA, 50% glycerol, 0.05% TRITON™ X-100 Detergent, pH 7.5.
Reconstitution
Following initial thaw, aliquot and freeze (-70°C).
Other Notes
Hug, J. and Sarre, T.F. 1993. Biochem. J. 291, 329.
Kazanietz, M.G., et al. 1993. Mol.Pharmacol. 44, 298.
Koide, H., et al. 1992. Proc. Natl. Acad. Sci. USA 89, 1149.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow
Assay: ≥. 95% (SDS-PAGE). Quality Level: 100. form: liquid. specific activity: ≥. 800 . units/mg protein. manufacturer/tradename: Calbiochem®. . storage condition: OK to freeze, avoid repeated freeze/thaw cycles. shipped in: wet ice. storage temp.: −. 70°C. Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.- UPC:
- 51182439
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 539672-5UG
