PLASMIN FROM HUMAN PLASMA

General description

Inhibitors: DFP, PMSF, TLCK, leupeptin, aprotinin, soybean trypsin inhibitor, α₁-antitrypsin, α₂-macroglobulin, anti-thrombin III heparin, APMSF, and α₂-antiplasmin

Fibrinolysin

Specificity

Serine endopeptidase that hydrolyzes peptide and ester bonds at the carboxylic side of Lys or Arg; more selective than trypsin.

Application

Human plasmin has been used as an oligopeptide substrate in peptidase inhibition assays.

Biochem/physiol Actions

Plasmin is a direct acting fibrinolytic enzyme present in blood. Its activity is independent of plasminogen presence during fibrinolysis. Plasmin is rapidly and irreversibly inhibited by α-2 antiplasmin.

Physical form

Suspension in 3.2 M ammonium sulfate solution

Preparation Note

Stabilizers: Human plasmin does not contain stabilizers.
Storage conditions (working solution): 2 to 8 °C
Diluted solution (pH 8.6) is stable at 2 to 8 °C for at least 1–2 weeks. For storage of dialyzed solution freezing in aliquots is recommended.

Storage and Stability

Store at 2 to 8 °C. (Recommended form for storage is ammonium sulfate suspension 3.2 M, pH 8.6.)

Other Notes

For life science research only. Not for use in diagnostic procedures.