General description
Highly purified preparation of an altered form of human PAI-1 containing four mutated amino acids. Mutant PAI-1 is virtually unable to go latent and is stable at elevated temperature and pH for extended periods of time (t½ = 145 h at 37°C, pH 7.4). Inhibits uPA (Ki = 5.1 x 106 M-1sec-1) and tPA (Ki = 7.9 x 105 M-1sec-1).
Highly purified preparation of an altered form of human PAI-1 containing four mutated amino acids. Mutant PAI-1 is virtually unable to go latent and is stable at elevated temperature and pH for extended periods of time (t½ = 145 hours at 37°C, pH 7.4). Inhibits uPA (Ki = 5.1 x 106 M-1 sec-1) and tPA (Ki = 7.9 x 105 M-1 sec-1).
Biochem/physiol Actions
≥90% by uPA assay.
Cell permeable: no
Primary Target
Plasminogen Activator
Product does not compete with ATP.
Reversible: no
Target Ki: 5.1 x 106 M-1 sec-1, 7.9 x 105 M-1 sec-1 against uPA and tPA, respectively
Packaging
Please refer to vial label for lot-specific concentration.
Warning
Toxicity: Standard Handling (A)
Physical form
In 100 mM NaCl, 50 mM sodium phosphate buffer, 1 mM EDTA, pH 6.6.
Reconstitution
Following initial thaw, aliquot and freeze (-70°C).
Other Notes
Berkenpas, M.B., et al. 1995. EMBO J. 14, 2969.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Quality Level: 100. Assay: ≥. 95% (SDS-PAGE). form: liquid. manufacturer/tradename: Calbiochem®. . storage condition: OK to freeze, avoid repeated freeze/thaw cycles. shipped in: wet ice. storage temp.: −. 70°C. Storage Class Code: 10 - Combustible liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.- UPC:
- 12352200
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 528208-50UG