PLC catalyzes the hydrolysis of phosphatidyl inositol (4,5) bisphosphate (PIP2) on the cytoplasmic side of the cell membrane to yield diacylglycerol and inositol-1,4,5-triphosphate (IP3). This reaction initiates a well-known signal transduction pathway common to most cells. PLC thio-PIP2 is an analog of naturally occurring PIP2 that contains sulfur rather than oxygen at the sn-3 position of the glycerol backbone. Hydrolysis by PLC yields a free thiol which reacts with chromogenic reagents such as DTNB (Ellman’s reagent) to allow quantitation of PLC activity.
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- UPC:
- 41116113
- Condition:
- New
- Availability:
- 3 Days
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 10007839-100
- Model Number:
- C835A03
- Temperature Control Device:
- Yes