General description
Poly(A) is used as a carrier for quantitative precipitation of DNA and RNA.
Application
Poly(A) has been used for droplet digital PCR (ddPCR) assay.
Polyadenylic acid (Poly(A)) to inhibit Exo1′s exonuclease activity. It has also been used as a carrier to resuspend lyophilized gBlocks.
Biochem/physiol Actions
Polyadenylic acid (Poly(A)) tails present at 3′ end are produced in the cell nucleus. It contains ~250 nucleotides in mammalian cells. Poly(A) regulates mRNA decay and the initiation of translation. Cytoplasmic poly(A) extension modulates translation.
Quality
Typical analysis:
2.3μmol/mg Poly(A) (from absorbance) in relation to one mononucleotide unit. Chromatographically homogeneous.
Absorbance determination A250/A260, A280/A260, A290/A260
Sequence
Chain Length 2.100 to 10.000 nucleotides
Unit Definition
1 A260 unit corresponds to 40 μg ssRNA.
Physical form
Lyophilizate, potassium salt
Preparation Note
Working concentration: 0.5 mg/ml
A concentration of 0.5 mg/ml is recommended.
Working solution: 0.5 mg/ml is recommended.
Other Notes
For life science research only. Not for use in diagnostic procedures.
- UPC:
- 12352100
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 10108626001