Poly-D-lysine hydrobromide (C09-1019-804)

Poly-D-lysine hydrobromide is a positively charged synthetic polyamino acid having one HBr per lysine unit. It is a crystalline solid soluble in water. Poly-D-lysine hydrobromide is widely used as a coating to enhance cell attachment and adhesion to both plasticware and glass surfaces. This polycation is resistant to enzymatic degradation and has been used to culture a wide variety of cell types.Poly-D-lysine hydrobromide with a molecular weight greater than 30,000 Da is recommended for cell culture applications. The poly-D-lysine hydrobromide (MW=52,000 Da) is easier to use because it is less viscous in solution, however the poly-D-lysine hydrobromide with an higher molecular weight (MW=84,000 Da) has more attachement sites per polymer chain available to the cells. Both poly-D-lysine hydrobromide and poly-L-lysine hydrobromide can be used as a coating substrate, however certain cells digest poly-L-lysine hydrobromide. In this case, poly-D-lysine, as the attachment factor, is necessary to prevent cell disruption by excessive uptake of L-lysine.Our poly-D-lysine hydrobromide has been purified by dialysis, sterile-filtered on 0.2um, lyophilized, and stored under Ar. Specification: Mn~84000 Da by NMR (equivalent to Mw 150-300 kDa by viscosity ) MDL Number: MFCD00131934 PubChem CID: 87493163 Isomeric SMILES: C(CCN)C[C@H](C(=O)O)N.Br Related Documents: https://ald-pub-files.oss-cn-shanghai.aliyuncs.com/aladdinsci/pdp/sds/1/P477809-SCI_77d5d96bc260624210190f627abfd3d7.pdf