Poly(ethylene glycol)-block-poly(propylene glycol)-block-poly(ethylene glycol) diacrylate

Polyether F127 diacrylate (P477925) is a double bond modified polyethylene glycol polypropylene glycol polyethylene glycol triblock copolymer, which can be quickly crosslinked and cured into a gel by UV and visible light under the action of photoinitiators. F127 has excellent thermotropic gel (heating gel) characteristics and good biosafety. The material system based on F127 can be used in the biomedical field, such as as drug carrier, wound dressing, cell carrier shear protector, biological 3D printing, etc.Application:Polyethylene glycol block polypropylene glycol block polyethylene glycol diacrylate (PEG-PPG-PEG) is an amphiphilic triblock copolymer based on bifunctional acrylate, which can be used in pharmaceuticals, biopharmaceuticals, and personal care products. It can also be used for synthesizing alternating block polyurethanes for drug delivery.Storage and transportation:Dry set: room temperature, 3 months; 4 ℃, 12 months- 20 ℃, 18 months. Sterile solution: protected from light at 4 ° C for 7 days- Avoid light at 20 ℃ for 6 months. Repeated freezing and thawing of the solution can affect product performance, so it is recommended to prepare and use it as soon as possible.Solution preparation:1. Prepare a 0.25% (w/v) initiator standard solution(1) Take 20mL PBS and add it to a brown bottle containing initiator LAP (containing 0.05g of LAP);(2) Heat and dissolve in a 40-50 ℃ water bath for 15 minutes, during which manually or vortex oscillate 2-3 times for 5-10 seconds each time.This LAP standard solution can be stored for 12 months under dark conditions at 4 ° C.2. Prepare P477925 solution (the recommended concentration of P477925 is 5~30% w/v, and below 5% is suitable for combination with other hydrogels for 3D cell culture)(1) Take the required mass of P477925 and place it in a centrifuge tube.(2) Take the standard solution of the initiator and add it to the centrifuge tube mentioned above.(3) Dissolve at 2-8 ℃ for 30 minutes, with 2-3 manual or vortex oscillations for 5-10 seconds each time (concentration<20% can be dissolved by stirring at room temperature).Curing use:Irradiate the above P477925 solution containing initiator with 405nm light source, and it can be cured into gel within 10-30s. In order to obtain higher strength hydrogel, the light duration can be extended as appropriate.Explanation:P477925 solution has thermally induced gel characteristics (heating up gel). During solution preparation, cooling is conducive to dissolution. It is recommended to let the solution dissolve at 2-8 ℃ and shake several times with a vortex mixer during this period.P477925 solution with a concentration ≥ 20% will appear gel when it stands at room temperature. The higher the concentration is, the easier it is to gel. At this time, the gel is a physically reversible gel. When the temperature drops to 2~8 ℃, the solution will resume its state.Kind reminder: Do not look directly at the curing light source. Semi finished kit:Component A: F127DA, white powder, 1g/bottle or 5g/bottle: stored in a dark placeComponent B: Photoinitiator LAP, white powder, 0.05g/bottle * (1 or 5): stored in a dark place. Specifications and Purity: average Mₙ ~15kDa. Molecular Formula: C3H3O[C2H4O]x[C3H6O]y[C2H4O]zC3H3O2.