POLY-L-LYSINE HYDROBROMIDE MOL WT 70 000-150 000 BIOREAGENT G-IRRADIATED LYOPHILIZED POWDER SUITABLE FOR CELL CULTURE (C005B-438732)

Application

Poly-L-lysine hydrobromide has been used to precoat glass bottom dishes used for in vitro cell culture.

Recommended as a cell culture substratum when using 0.5 ml of a 0.1 mg/ml solution to coat 25 cm². Optimal conditions for attachment must be determined for each cell line and application.

Components

Poly-L-lysine is a positively charged amino acid polymer with approximately one HBr per lysine residue. The hydrobromide allows the poly-L-lysine to be in a crystalline form soluble in water. A small amount of product may be found in the beta structure because the HBr interferes with hydrogen bonding between amino and either the carboxyl groups or N or O containing moieties.

Caution

Sterile solutions are stable for up to 2 years when stored at 2-8°C. It should be stored desiccated at -20°C.

Analysis Note

Molecular weight based on viscosity. Also assayed by MALLS.

Other Notes

For additional technical information on polyamino acids please visit the Polyamino acid FAQ resource.