Poly-L-Ornithine Solution (0.01%) (C15-1314-763)

General description

Poly-L-Ornithine is a synthetic amino acid chain that is positively charged having one hydrobromide per unit of ornithine. Poly-L-Ornithine is widely used as a coating to enhance cell attachment and adhesion to both plasticware and glass surfaces. Poly-L-Ornithine is often used in combination with laminin for many types of neuronal and neural stem cell cultures.

The molecular weight of Poly-L-Ornithine for cell culture can vary significantly, with lower molecular weight (30,000 Da) being less viscous and higher molecular weight (greater than 300,000 Da) having more binding sites per molecule. This product uses a Poly-L-Ornithine of 78000 Da, yielding a solution viscosity for easy handling while providing sufficient binding sites for cell attachment.

Application

Optimal coating concentrations must be determined by end user. Typical coating concentrations range from 10ug/ml to 100ug/ml based on cell type and application.

1. Thaw Poly-L-Ornithine solution at room temperature.
2. Dilute Poly-L-Ornithine solution to desired concentration in sterile water.
3. Fully coat the cell culture surface with diluted Poly-L-Ornithine solution. Use 5 mL volume for 6-cm plates and 10 mL volume for 10-cm plates and T75 flasks.
4. Allow cell culture vessel to sit at room temperature overnight.
5. Aspirate the Poly-L-Ornithine solution the following day and rinse vessel with sterile water followed by coating with desired ECM protein.

Research Category
Stem Cell Research

Research Sub Category
Adhesion (CAMs)

ECM Proteins

Quality

Solution is clear with no particulates present. Solution is sterile and suitable for cell culture applications.

Physical form

Product is presented in sterile water at 0.01% w/v (0.1mg/ml). Product is filtered through a 0.2 micron filter before freezing.

Storage and Stability

Store at -20°C for up to 4 months from date of receipt. Avoid multiple freeze thaw cycles to maintain product integrity.

Legal Information

NOVASEPTUM is a registered trademark of Millipore AB - Sweden

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

needle size: 2.0 mm. Quality Level: 100. sterility: sterile. form: liquid. mol wt: M_w 78000 . Da. manufacturer/tradename: NovaSeptum^®. . concentration: 0.01 . % (w/v), 0.1 . mg/mL. technique(s): cell attachment: suitable, cell culture | mammalian: suitable. Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.