Polyclonal Antibody to Human Topoisomerase II Alpha

Product InfoPolyclonal Antibody Unit Definition:The antibody concentration is in Western blotting units; one unit corresponds to a 1:1000 dilution of the antibody required to make 1 ml of diluted probe for a routine Western blot. Thus, 10 units of antibody will make 10 ml of diluted probe.Material safety data:THIS PRODUCE IS NOT LICENSED OR APPROVED FOR ADMINISTRATION TO HUMANS OR TO ANIMALS OTHER THAN EXPERIMENTAL ANIMALS.The product is sold as an antibody for research purposes only. Safe lab practices should be employed when handling this product. Chemical, physical and toxicological analyses of the product have not been performed.DescriptionThis vial contains a 16 residue oligopeptide derived from the carboxy terminal region of human topoisomerase IIa.  The purity of the peptide was greater than 95% as determined by HPLC and mass spectral analysis.  Approximately 10 mg of purified peptide were coupled (through a terminal cysteine thiol) to KLH with a heterobifunctional cross-linking agent MBS at a ratio of 1 part peptide to 1 part KLH.  The peptide was used to immunize rabbits for antibody production. The resulting antibody reacts strongly and specifically with the 170 kDa Human Topo II protein.  This peptide is used as a control reagent in immunofluorescence; it effectively neutralizes the antibody to topo II.A polyclonal antibody was prepared against a 16 residue oligopeptide derived from the carboxy terminal region of human topoisomerase II. The purity of the peptide was greater than 95% as determined by HPLC and mass spectral analysis. The antibody titer was determined by ELISA to be > 1:104. Immunoaffinity gel chromatography and antibody purification steps were carried out to obtain a homogeneously purified IgG.This antibody is ideal for either Western blotting applications or immunoflourecence (IF) studies on the cytological localization of topoisomerase II. The dilutions of antibody required for specific detection should be optimized for the investigator’s particular system (cell line being used for example); however, we supply the antibody reagent along with a detailed protocol for immunofluorescence using a typical human cell line (optimized for HeLa cells). A second reagent, the 16 -mer oligopeptide ( TG2011-2) is also available. This allows the investigator to perform proper controls for absorbing the antibody to demonstrate specificity in IF experiments. The IF protocol has been optimized for tumor cells that possess relatively high levels of topoisomerase II. In addition, we offer a Western blot marker set for topo II (TG2011-3) to unambiguously locate the position of the 170 kDa topo II. Related Document: https://ald-pub-files.oss-cn-shanghai.aliyuncs.com/aladdinsci/pdp/sds/1/P419216-SCI_d720452dde8a18f705070fed399b6a71.pdf.