General descriptionPolyethylene glycol (PEG) is a polymer of ethylene oxide and water, obtained upon condensation. ApplicationPolyethylene glycol solution has been used in the hybridization of the following: 1, irradiated camel fibroblast cells with A23 hamster cells2, mouse splenocytes with Sp2/0-Ag 14 mouse myeloma cells3, rabbit splenocytes and cell line 240E-1Recommended for use in a normal fusion protocol requiring 50% PEG.PEG is used as a fusogen to obtain hybridomas for monoclonal antibody production. Induces cell hybridization.Other NotesContains 50% (w/v) polyethylene glycol (Av. Mol. Wt. 1450) in DPBS without calcium.ReconstitutionSolution is ready-to-use. If a less concentrated solution is desired, dilute with sterile DPBS without calcium (D5773). Some precipitate may appear after being exposed to cooler, but should disappear as the solution warms. Solution may be frozen if desired but should first be aliquotted to avoid repeated freeze/thaw cycles. Specification: 50 % (w/v), average mol wt 1,450, sterile-filtered, BioReagent, suitable for hybridoma Molecular Formula: HO(CH2CH2O)nH Molecular Weight: average Mn 4000
- UPC:
- 12352137
- Condition:
- New
- Availability:
- 8-12 weeks
- Weight:
- 1.23 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- P615453-5ml
- CAS:
- 25322-68-3
- Product Size:
- 5ml
