POLYNUCLEAR AROMATIC HYDROCARBONS (C005B-227523)

General description

Phosphohistone H3 (PHH3) is a core histone protein, which together with other histones, forms the major protein constituents of the chromatin in eukaryotic cells. In mammalian cells, phosphohistone H3 is negligible during interphase but reaches a maximum for chromatin condensation during mitosis. Immunohistochemical studies showed anti-PHH3 specifically detected the core protein histone H3 only when phosphorylated at serine 10 or serine 28. Studies have also revealed no phosphorylation on the histone H3 during apoptosis. Therefore, PHH3 can serve as a mitotic marker to separate mitotic figures from apoptotic bodies and karyorrhectic debris, which may be a very useful tool in the diagnosis of tumor grades, especially in CNS, skin, gyn., soft tissue, and GIST.

Quality

IVD

IVD

IVD

RUO

Linkage

Phosphohistone H3 (PHH3) Positive Control Slides, Product No. 369S, are available for immunohistochemistry (formalin-fixed, paraffin-embedded sections).

Physical form

Solution in Tris Buffer, pH 7.3-7.7, with 1% BSA and <0.1% Sodium Azide

Preparation Note

Download the IFU specific to your product lot and formatNote: This requires a keycode which can be found on your packaging or product label.

Other Notes

For Technical Service please contact: 800-665-7284 or email: service@cellmarque.com

Legal Information

Cell Marque is a trademark of Sigma-Aldrich Co. LLC

biological source: rabbit. Quality Level: 100, 500. conjugate: unconjugated. antibody form: Ig fraction of antiserum. antibody product type: primary antibodies. clone: polyclonal. description: For In Vitro Diagnostic Use in Select Regions (See Chart). form: buffered aqueous solution. species reactivity: human. packaging: vial of 0.1 . mL concentrate (369A-14), vial of 0.5 . mL concentrate (369A-15), bottle of 1.0 . mL predilute (369A-17), vial of 1.0 . mL concentrate (369A-16), bottle of 7.0 . mL predilute (369A-18). manufacturer/tradename: Cell Marque^™. . technique(s): immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100-1:500. control: tonsil. shipped in: wet ice. storage temp.: 2-8°C. visualization: nuclear. Gene Information: human ... H3C1(8350).