Potassium Ferrocyanide Trihydrate, 500g

Potassium ferrocyanide trihydrate is a salt of the Fe(CN)64- ferrocyanide ion. Ferrocyanide is a reducing iron species, being oxidized from ferrous Fe(II) to ferric Fe(III). Potassium ferrocyanide is used to determine the concentration of potassium permanganate, a compound often used in titrations based on redox reactions. Potassium ferrocyanide is used in a mixture with potassium ferricyanide and phosphate buffered solution (PBS) to provide a buffer for X-Gal, which is used to cleave Beta-galactosidase, giving a bright blue visualization where an antibody (or other molecule), conjugated to Beta-gal, has bonded to its target. The ferrocyanide species is membrane impermeable and can be used to assess redox activity of membrane-bound structures and mechanisms of electron transport across membranes. This approach was employed to demonstrate that Potassium ferrocyanide performs reducing activation of membrane-bound dopamine β-monooxygenase from the exterior face of adrenal vesicles, providing elucidation of native reduction-activation mechanisms for dopamine β-monooxygenase regulation.