Powdered, TC-100 Insect Medium with L-Glutamine, without Sodium bicarbonate. Pack Size Represents Final Volume When Hydrated. Pack size is 20L

TC-100 is a modification done by Dr. Gardiner and Dr. Stockdale of the original formulation of Grace's insect medium. Sucrose, fructose and several Kreb's cycle intermediates were omitted from the original formula. The modification was done to optimize the production of Autographia californica NPV virions by cells from the fall armyworm, Spodoptera frugiperda. IM007 contains L-glutamine and does not require supplementation with insect hemolymph. Instead, tryptose broth and fetal bovine serum provide the necessary growth factors. This medium supports the growth of several lepidopteran cell lines including Spodoptera frugiperda. It is specifically used for production of baculoviruses in lepidopteran cell lines. Appearance: Off-white to Creamish white, homogenous powder Solubility: Clear solution at 20.0gms/L pH without Sodium Bicarbonate 3.90 -4.50 pH with Sodium Bicarbonate 4.70 -5.30 Osmolality without Sodium Bicarbonate 220.00 -260.00 Osmolality with Sodium Bicarbonate 230.00 -270.00 Cultural Response: The growth promotion capacity of the medium is assessed qualitatively by analyzing the cells for the morphology and quantitatively by estimating the cell counts and comparing it with a control medium through minimum three subcultures Endotoxin Content: NMT 5EU/ml