Powdered, TNM-FH Insect Medium with L-Glutamine, without Sod (C08-0447-653)

TNM-FH medium is a modification of Grace's insect medium. The modification was developed by Dr. W. F. Hink to grow Cabbage looper cell line TN-368. Presently, this medium is used to grow cells derived from a variety of lepidopterans species. IM008 is supplemented with lactalbumin hydrolysate and yeast extract. Lactalbumin hydrolysate provides a large number of free amino acids. Yeast extract serves as a source of vitamins. Supplemented with 5-20% fetal bovine serum this medium supports growth of number of insect cell lines derived from Lepidopteran species. Appearance: Off-white to Creamish white, homogenous powder Solubility: Solution with some particles at 52.4gms/L pH without Sodium Bicarbonate 4.00 -4.60 pH with Sodium Bicarbonate 4.30 -4.90 Osmolality without Sodium Bicarbonate 335.00 -375.00 Osmolality with Sodium Bicarbonate 340.00 -380.00 Cultural Response: The growth promotion capacity of the medium is assessed qualitatively by analyzing the cells for the morphology and quantitatively by estimating the cell counts and comparing it with a control medium through minimum three subcultures Endotoxin Content: NMT 15EU/ml