Premium-Grade EDC (C09-1510-620)

Product introduction:Premium Grade EDC is our highest quality formulation of this popular carbodiimide crosslinker, specially characterized for applications where product integrity and risk minimization are paramount.Features of Premium Grade EDC:• :EDC:—carbodiimide crosslinker for conjugation between carboxylates and amines:• :High quality:—identity and purity confirmed by several tests:• :Product integrity:—enhanced level of testing and characterization compared to standard grade:• :Lot retention:—ample supply of past lots retained to ensure future process testing:• :Change management:—Change Control Notification (CCN) service:• :Consistent manufacture:—batch-specific manufacturing documentation review:Compared to the standard grade product, Premium Grade EDC provides more clearly defined quality and product support by including (a) increased analytical testing and product characterization, (b) greater batch-specific information and quality assurance review, (c) extensive lot sample retention and (d) change control notification. EDC is 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride, a water-soluble carbodiimide crosslinker that activates carboxyl groups for spontaneous reaction with primary amines, enabling peptide immobilization and hapten-carrier protein conjugation. In the presence of :N:-hydroxysulfosuccinimide (sulfo-NHS), EDC can be used to convert carboxyl groups to amine-reactive sulfo-NHS esters. This is accomplished by mixing the EDC with a carboxyl containing molecule and adding sulfo-NHS.Specifications of Premium Grade EDC:We manufacture crosslinking reagents to ensure the highest possible overall product integrity, consistency and performance for the intended research applications.• Visual: White crystalline powder, free of foreign matter:• Identity: IR scan shows only peaks characteristic of EDC:• Water content: Karl Fischer titration, ≤ 2%:• Trace metals: All metals, including lead, ≤ 50 ppm:• Elemental analysis: Reported values for C, H, and N:Applications:• Conjugate carboxyl and amino groups among peptides and proteins:• Couple haptens to immunogenic carrier proteins (e.g.,attach a peptide to KLH):•Immobilize peptide antigens to affinity-purify antibodies:• Create NHS-activated, amine-reactive labeling compounds:• Crosslink proteins to carboxyl coated beads or surfaces:• Activate nanoparticles with amine-reactive sulfo-NHS esters:Product References:Crosslinker Application Guide -- search for recent literature references for this product:• :Reactive Moiety:Carbodiimide :• :Crosslinker Type:Heterobifunctional :• :Form:Solid:• :Spacer:Short(<:10 Å) :• :Labeling Method:Chemical Labeling :• :Spacer Arm Length:0.0 Å Molecular Formula: C8H18ClN3 Molecular Weight: 191.70 EC Number: 247-361-2 PubChem CID: 2723939 Isomeric SMILES: CCN=C=NCCCN(C)C.Cl Beilstein Registry Number: 5764110