Primary Cancer Culture System

General description

Primary Cancer Culture System

Application

The Primary Cancer Culture System is a standardized, defined and animal component-free (D-ACF) system for the isolation and culture of human primary tumor cells. The medium was designed to be the first universally applicable in vitro cell culture solution for the isolation and long-term culture of primary human malignancies, e.g. from patient tumor samples or patient-derived xenografts (PDX). Due to precise stromal control, prolonged culture allows for functional selection of malignant cells. This principle enables access to an enriched population of primary cancer cells. The selection process dispenses with the use of toxic agents. Since on the cellular level malignancy itself is the only selection criterion, the cell diversity of the cancerous subpopulations of the original tumor is preserved.The Primary Cancer Culture System can also be used for other applications, e.g. enriching cell lines or depleting of stromal cells and other non-cancerous cells form established primary cancer cell cultures.Recommended for:Tumor tissue samplesMalignant / cancerous cellsEstablished primary tumor cell cultures

Features and Benefits

Perform up to five primary tumor isolations with one medium kit
Successfully prevent stroma overgrowth when isolating fresh tumor samples
Establish a culture of pure malignant cells within 4-6 weeks
Culture PDX tumor models in vitro
Defined and animal component-free formulation

Components

Primary Cancer Cell Medium D-ACF, 250ml
SupplementMix for 250ml
NCCD surface treatment reagent, 2ml

Note: The NCCD Reagent is intended for treatment of the culture surface. Do not add the NCCD Reagent to the medium.

Quality

All lots of PromoCell Primary Cancer Cell Culture Kit are subjected to comprehensive quality control tests using a human cancer cell line. Each lot is checked for growth promoting activity for malignant cells, adherence rate, and typical morphology of the test cell line. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).

Storage and Stability

Store the Basal Medium at 2 to 8°C in the dark and the SupplementMix at -20°C immediately after arrival. Do not freeze the Basal Medium. If stored properly, the products are stable until the expiry date stated on the label. After adding the supplements to the Basal Medium, the shelf life of the complete medium is 8 weeks at 2 to 8°C. Do not freeze the complete medium. For use, pre-warm only an aliquot of the complete medium and keep the remaining medium refrigerated at 2 to 8°C

Subculture Routine

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