PRIMER FOR CDNA SYNTHESIS P(DT)15

General description

This primer consists of a 15-mer oligonucleotide that shows thymidine (T) at each individual position. The oligonucleotides are HPLC purified, desalted, and supplied as a lyophilizate of the Li-salt. The p(dT)₁₅ primer is not phosphorylated at the 5′ end.Oligo(dT)N generates full-length cDNAs. If template contains oligo(A) stretches internally, the primer may bind these, causing mis priming and transcripts that are not full-length.

Composition: p(dT)₁₅[ 15-mer oligonucleotide containing only deoxythymidine (dT) residues].

Phosphorylation: The p(dT)₁₅ primer is not phosphorylated at the 5′ end.

Application

Primer for cDNA synthesis may be used in:

• RT-PCR
• Generation of cDNA libraries
• Synthesis of first-strand cDNA

Unit Definition

8 nmol ξ 1 A₂₆₀ unit

Other Notes

For life science research only. Not for use in diagnostic procedures.