Pro-survival Compound, DDD00033325 - Calbiochem (C15-1304-975)

General description

A cell-permeable, pyrrolidine small molecule that promotes single human embryonic stem cell survival up to 20-fold over background (EC₅₀ = 3.9 M), with equal or higher potency than Y-27632 (Cat. No. 688000) in 5 cell lines (SA461, SA181, SA167, SA121 and SA002) tested at 10 M. An in vitro high-content screening assay, which profiles a panel of more than 70 protein kinases, indicates that this small molecule is more selective than Y-27632 and only displays an inhibitory effect toward ROCK2 (>83% inhibition, ROCK1 was not included in the panel) and PRK2 (76% inhibition) at 10 M. Furthermore, it exhibits less cytotoxicity at a greater concentration of 30 M than Y-27632 without marked adverse effects for up to 120 hours.

A cell-permeable, pyrrolidine small molecule that promotes single human embryonic stem cell survival up to 20-fold over background (EC₅₀ = 3.9 M), with equal or higher potency than Y-27632 in 5 cell lines (SA461, SA181, SA167, SA121 and SA002) tested at 10 M. An in vitro high-content screening assay, which profiles a panel of more than 70 protein kinases, indicates that this small molecule is more selective than Y-27632 and only displays an inhibitory effect toward ROCK2 (>83% inhibition, ROCK1 was not included in the panel) and PRK2 (76% inhibition) at 10 M. Furthermore, it exhibits less cytotoxicity at a greater concentration of 30 M than Y-27632 without marked adverse effects for up to 120 hours.

Packaging

Packaged under inert gas

Warning

Toxicity: Regulatory Review (Z)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C. Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Andrews, P.D., et al. 2010. Biochem. J.432, 21.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Molecular Weight: 293.37. Empirical Formula: C₁₇H₁₉N₅. Quality Level: 100. Assay: ≥. 95% (HPLC). form: syrup. manufacturer/tradename: Calbiochem^®. . storage condition: OK to freeze, protect from light. color: yellow. solubility: DMSO: 100 . mg/mL. shipped in: wet ice. storage temp.: −. 20°C. SMILES string: C12: CC: NN1C: CC(N[C@@H]3CCN(CC4: CC: CC: C4)C3): N2. Storage Class Code: 10 - Combustible liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.