General description
ProbeQuant™ G-50 Micro Columns use spin-column chromatography to provide a fast and reliable method for purifying labeled probes from unincorporated labeled nucleotides.
ProbeQuant™ G-50 Micro Columns use spin-column chromatography to provide a fast and reliable method for purifying labeled probes from unincorporated labeled nucleotides. In addition, a portion of the eluted DNA can be counted in a scintillation counter and compared to an unpurified sample to give an approximation of percent incorporation. The columns are designed for use in a microcentrifuge, come prepacked with Sephadex® G-50 DNA grade and are pre-equilibrated in STE, which means that they are ready to use. They can accommodate samples ranging in volume from 25 μ to 50 μ.
Features and Benefits
- For rapid purification of labeled DNA (20 bases) from unincorporated labeled nucleotides in a volume of 25 to 50 μ using spin-column chromatography
- Convenient: Prepacked with Sephadex® G-50 DNA Grade (see illustra™ Sephadex® G-50 and G-100 DNA Grade) and pre-equilibrated in STE containing 0.15% Kathon CG/ICP Biocide
- Ready to use: Requires less than 4 min from sample application to collection of purified product
- Designed for use in a microcentrifuge
- Tested in nickase, single and double-stranded exonuclease and RNase assays
Storage and Stability
Store at 4 to 30 °C ()
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Legal Information
ProbeQuant is a trademark of Cytiva
Sephadex is a registered trademark of Cytiva
illustra is a trademark of Cytiva
- UPC:
- 41115831
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- GE28-9034-08