Biochem/physiol Actions
Propidium monoazide (PMA) is a propidium derivative with an azide group that allows crosslinkage to DNA upon light exposure. PMA is only permeable to bacteria with compromised membrane integrity, making it highly selective for sick/dying/dead cells over healthy cells. PMA is typically added to cell samples (50 μM for 1-15 min in the dark) prior to cross-linking induction (650-W halogen light source), washing and DNA extraction. Due to its higher charge, PMA (+2) is less permeant to live cells and more selective than ethidium monoazide/EMA (+1) for dead cells. The stained cells can also be viewed and analyzed by fluorescence microscopy.
- UPC:
- 51282812
- Condition:
- New
- Availability:
- 3-5 Days
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- MPN:
- SML3448-5MG
- CAS:
- 91416-20-5
