Prostaglandin E2, Biotrak(TM) EIA

General description

Prostaglandin E₂ Direct Biotrak^™ Assay.

Application

Prostaglandin E2 (PGE2) competitive Biotrak^™ system enzymeimmunoassay system from Cytiva is specifically designed for research purposes. RPN222 includes protocols using novel lysis reagents in order to facilitate simple and rapid extraction of PGE2 from cell cultures and plasma samples. These components obviate the need for removal of extracting reagents prior to measurement, ensuring PGE2 is directly available for subsequent analysis. With the method for measurement of PGE2 in plasma, complex, time-consuming sample preparation procedures are avoided. Each pack of RPN222 contains sufficient material for 96 wells. This allows the construction of one standard curve and the measurement of 37 unknowns in duplicate. Each pack of RPN22210 contains sufficient material for 960 wells. This allows for the construction of one standard curve and the measurement of 37 unknowns in duplicate per plate.


Lysis reagent 1 hydrolyses cell membranes to release intracellular PGE2, or, dissociates PGE2 from soluble receptors and interfering binding Proteins present in plasma. Lysis reagent 2 sequesters the key component in lysis reagent 1 and ensures PGE2 is free for subsequent analysis. The detergent/sequestrant complex does not interfere with antigen: antibody binding. Lysis reagent 1 is simply added to cultured cells or plasma samples, followed by a ten min incubation before assay. The antisera and PGE2 peroxidase conjugate are reconstituted with lysis reagent 2. The assay is based on competition between unlabeled PGE2 and a fixed quantity of peroxidase-labeled PGE2 for a limited number of binding sites on a PGE2-specific antibody.

Features and Benefits

• Specific for PGE2.
• Improved technology uses novel lysis reagents that are proprietary to Amersham^™ Biosciences (lysis reagent 1 and lysis reagent The lysis reagents eliminate time-consuming extraction procedures.
• Flexible method – choice of five assay protocols.
• Direct measurement of PGE2 in plasma.
• Dual-range 1 to 32 pg/well (2°C–8°C protocol).
• Precise and accurate measurement.
• Ready-to-use substrate.
• Color-coded reagents.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

Biotrak Prostaglandin E2
The use of this product in a binding assay is the subject of patent numbers US 6,900,019 and EP 0863402 and equivalent patents and patent applications in other countries in the name of Cytiva UK Limited.

Amersham is a trademark of Cytiva

Biotrak is a trademark of Cytiva