Protease Inhibitor Cocktail Kit

Protease Inhibitor Cocktail Kit is a broad spectrum protease inhibitor cocktail which covers both endopeptidases and exopeptidases, non-specific and specific. The cocktail consists of four major protease inhibitors: AEBSF, Sodium EDTA, Leupeptin and Pepstatin A. Protease Inhibitor Cocktail Kit contains inhibitors with a broad specificity for serine, cysteine, and acid proteases, and aminopeptidases. It has been tested on a wide variety of cells and tissue extracts to isolate/maintain intact proteins without degradation. Place the contents of all four vials in one volumetric flask or other suitable container. Add water or buffer of choice to 100 mL. More concentrated solutions can be prepared as desired; however, leupeptin and pepstatin A will create more of a suspension at concentrations other than the suggested amount. The prepared solution is stable for 60-90 days when stored at 5 °C. Do not freeze the prepared solution, and avoid repeated freeze-thaw cycles. One mL of the cocktail solution is recommended for the inhibition of proteases equivalent to 1 mg of USP pancreatin. One kit is recommended for the inhibition of proteases present in a maximum of 20 g of cell extract. Since not all organisms contain the same amount of endogenous proteases it may sometimes be necessary to increase the concentration of inhibitors. Typical use is 1 mL of cocktail per 1 mL of sample.