General description
protein A HP SpinTrap™ are designed for the enrichment of proteins of interest, immunoprecipitation and the purification of monoclonal and polyclonal antibodies from cell lysates and biological fluids. Contain protein A Sepharose™ HP.
Application
Protein A Sepharose™ HP has high affinity for the Fc region of IgG from a variety of species. The two options in the Protein enrichment protocol allow the attached antigen (Protein of interest) to be eluted separately (cross-link protocol) or together with the antibody (classic protocol). There is also a protocol for efficient, small-scale purification of monoclonal and polyclonal antibodies from serum and cell culture supernatants in less than 20 min. No pretreatment of the sample is required.
There is also a protocol for efficient, small-scale purification of monoclonal and polyclonal antibodies from serum and cell culture supernatants in less than 20 min. No pretreatment of the sample is required.
Features and Benefits
- For simple, small-scale antibody purification or enrichment of specific Proteins in prepacked, single-use spin columns that are ready to use.
- Classic and cross-link protocols provide flexibility.
- Elution conditions formatted for both electrophoresis and LC-MS analysis workflows.
- Fast binding kinetics and high capacity provide high yield.
- Prepacked with Protein A Sepharose™ High Performance for coupling of antibodies of IgG subclasses.
- Easy scale-up with HiTrap® Protein A HP prepacked columns.
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Legal Information
HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva
SpinTrap is a trademark of Cytiva
- UPC:
- 41106300
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- GE28-9031-32